Phanta Max Tremendous-Fidelity DNA Polymerase (P505)

Phanta Max Tremendous-Fidelity DNA Polymerase can be a highly successful and accurate enzyme made for purposes that require substantial-fidelity DNA amplification. This polymerase is perfect for use in PCR, cloning, and also other molecular biology procedures exactly where precision and reliability are paramount.

Essential Features
1. Super-Fidelity

Overview: Phanta Max Super-Fidelity DNA Polymerase gives Extraordinary precision, significantly reducing the error price throughout DNA amplification.
Advantage: This substantial-fidelity attribute is important for programs for example cloning, mutagenesis, and sequencing, the place even minimal problems can cause substantial issues.
two. Significant Performance

Overview: The enzyme displays substantial processivity, enabling fast and effective DNA synthesis.
Benefit: This brings about shorter reaction times and better yields, building the polymerase appropriate for demanding and time-delicate initiatives.
3. Strong Effectiveness

Overview: Phanta Max is formulated to offer dependable performance throughout an array of templates and disorders.
Reward: It ensures dependable benefits, even with advanced or GC-wealthy templates, maximizing the versatility of the experiments.
four. Extended Fragment Amplification

Overview: Effective at amplifying extended DNA fragments nearly 40 kb from genomic DNA.
Gain: This capability is particularly practical for programs necessitating the amplification of huge genomic regions.
Polymerase Chain Response (PCR)

Use Case: Amplifying DNA for different downstream apps such as cloning, sequencing, and Evaluation.
Edge: The high-fidelity and efficiency of Phanta Max guarantee exact and strong PCR results.
Cloning and Mutagenesis

Use Situation: Creating precise DNA fragments for cloning into vectors or building specific mutations.
Benefit: Minimizes the chance of glitches all through amplification, supplying trustworthy constructs for more experiments.
Following-Era Sequencing (NGS)

Use Situation: Planning libraries for NGS.
Edge: Substantial-fidelity amplification lessens the introduction of sequencing glitches, improving upon details quality.
Gene Synthesis

Use Case: Synthesizing extensive and sophisticated genes for useful scientific tests or therapeutic apps.
Benefit: The polymerase’s capability to amplify long DNA fragments ensures the thriving synthesis of enormous genes.
Protocol and Usage
one. Response Setup

Template DNA: Ordinarily ten ng for plasmid or one hundred ng for genomic DNA.
Primers: 0.2 µM Each individual.
dNTPs: two hundred µM Each and every.
Phanta Max Super-Fidelity DNA Polymerase: one unit for every 50 µL response.
Buffer: Provided With all the enzyme, make sure proper focus as per company’s Guidelines.
MgCl₂: Included phanta max super-fidelity dna polymerase in the buffer or included individually if specified.
two. Thermal Cycling Conditions

First Denaturation: 95°C for three minutes.
Denaturation: 95°C for thirty seconds.
Annealing: fifty five-65°C for thirty seconds (enhance dependant on primer Tm).
Extension: 72°C for one minute for each kb of target.
Ultimate Extension: seventy two°C for 5 minutes.
Cycle Number: Generally twenty five-35 cycles, dependant upon template complexity and abundance.
three. Optimization Ideas

Template Quality: Ensure the DNA template is of superior purity to prevent inhibitors which could have an effect on the polymerase’s general performance.
Primer Design and style: Use primers which has a Tm inside of 2-three°C of one another for optimum annealing.
Mg²⁺ Focus: Regulate Mg²⁺ concentration if required to phanta max super-fidelity dna polymerase improve generate and specificity.
Phanta Max Super-Fidelity DNA Polymerase (P505) is an advanced Device for top-precision DNA amplification. Its Tremendous-fidelity, performance, and robust functionality enable it to be perfect for a wide range of molecular biology applications, making certain reputable and precise success. Regardless if you are engaged on cloning, gene synthesis, or next-generation sequencing, Phanta Max provides the precision and dependability essential to accomplish your investigate goals.

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